This movie demonstrates the combined recording of a z-stack with optical trapping and manipulation of a polystyrene bead in JPK's NanoTracker™ optical tweezers system. It shows an animated view of the 3D-reconstruction of a confocal z-stack. A bead is optically trapped and held at a fixed position relative to the sample chamber. The z-positioning system of the NanoTracker™ allows parallel z-movement of the sample and the trapped bead during the recording. With this, the relative position of the particle and
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